Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: ( A–F ) Representative Western blots and quantitative analysis for GRP78, ATF6, p-PERK, PERK, p-IRE1-a, IRE1-a. GAPDH was used as the internal control. ( G ) Representative H&E staining left ventricular sections (scale bar = 50μm μm). ( H ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. sham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Western Blot, Control, Staining

Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: ( A−F ) Representative Western blots and quantitative analysis for GRP78, ATF6, p-PERK, PERK, p-IRE1-a, IRE1-a. GAPDH was used as the internal control. ( G ) Representative H&E staining left ventricular sections (scale bar = 50μm μm). ( H ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. sham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Western Blot, Control, Staining

Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: CIRP and marker proteins amount of endoplasmic reticulum stress was significantly decreased after NaHS treatment:( A-–G ) Representative Western blots and quantitative analysis for CIRP, GRP78, ATF6, p-PERK, PERK, p-IRE1-a, and IRE1-a. GAPDH was used as the internal control. ( H ) Representative H&E staining left ventricular sections (scale bar = 50 μm). ( I ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. Ssham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Marker, Western Blot, Control, Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: DR5-mediated DISC controls caspase-8 cleavage and initiation of apoptosis in human glioblastomas

doi: 10.1111/j.1582-4934.2009.00777.x

Figure Lengend Snippet: Selective knockdown of RIP, c-FLIP and PED/PEA-15 sensitizes the resistant cells to TRAIL. (A) D247MG and U118MG cells were transfected with RIP, c-FLIP, PED/PEA-15 siRNA and scramble siRNA for 72 hrs. The expression of RIP, c-FLIP and PED/PEA-15 was examined by Western blots. (B) The cleavage of caspases and DFF45 was examined by Western blotting in the transfected cells following treatment with 300 ng/ml TRAIL for 3 hrs. Proteins are indicated on the left, and the molecular weights of the proteins and cleavage products are on the right side of the panels. (C) Cell death was measured by the cell viability assay in the transfected cells after treatment with 300 ng/ml TRAIL for 24 hrs (mean ± S.E.M., n = 8).

Article Snippet: The antibodies used in the study included those against Flag M2 (Sigma-Aldrich, Saint Louis, MO, USA), DcR1 (Calbiochem, San Diego, CA, USA), DcR2 (Imgenex, San Diego, CA, USA), DR4 (Ab1-DR4, Diaclone, Besancon, France; Ab2-DR4, Chemicon, Danvers, MA, USA; Ab3, Imgenex), DR5 (pROsCI., Inc., Poway, CA, USA), FADD, caspase-8 (Medical & Biological Laboratories, Nagoya, Japan), c-FLIP (NF6 clone from Alexis), PED/PEA-15 (a kind gift from Dr. Francesco Beguinot, Naples, Italy), DNA fragmentation factor (DFF45) and caspase-3 (StressGen, Ann Harbor, MI, USA), acetyl-CoA carboxylase and phosphorylated and unphosphorylated I-κB (Cell Signaling Technology, Beverly, MA, USA), Src kinase Fyn (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and caveolin-1 (BD Biosciences, San Jose, California, USA).

Techniques: Knockdown, Transfection, Expressing, Western Blot, Viability Assay